1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained. 

2. Technical specifications 
Sensitivity: 0.025 ppb
Detection limit
Honey	0.025 ppb
Milk	0.05 ppb
Fish, shrimp and meat	0.025 ppb
Plasma and serum	0.025 ppb
Egg	0.05 ppb
Urine	0.125 ppb
Feed	0.1 ppb
 
Cross-reaction rate
Chloramphenicol	100%
Levonitro base	0.5%
Thiamphenicol	< 0.01%
Florfenicol	< 0.01%